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Samples for dating need to be converted into a form suitable for measuring the content; this can mean conversion to gaseous, liquid, or solid form, depending on the measurement technique to be used.Before this can be done, however, the sample must be treated to remove any contamination and any unwanted constituents.However, the open lattice structure of the hydroxyapatite makes it highly contaminated with carbonates from ground water.Removal of carbonate contaminants through dilute acid washing is also not applicable because hydroxyapatite is acid soluble.The protein, which is mostly collagen, provides strength and flexibility to the bone whereas the hydroxyapatite gives the bone its rigidity and solid structure.In theory, both organic and inorganic components can be dated.

In cases when the protein portion of the bone sample is not well preserved and have already degraded due to warm conditions and fungal or bacterial attack, AMS dating labs carbon date individual amino acids to check if several of them give the same radiocarbon age.

It is also used in radiocarbon dating to calibrate radiocarbon ages.

New growth in trees occurs in a layer of cells near the bark.

In his Trattato della Pittura (Treatise on Painting), Leonardo da Vinci was the first person to mention that trees form rings annually and that their thickness is determined by the conditions under which they grew. S., Alexander Catlin Twining (1801–1884) suggested in 1833 that patterns among tree rings could be used to synchronize the dendrochronologies of various trees and thereby to reconstruct past climates across entire regions.

During the latter half of the nineteenth century, the scientific study of tree rings and the application of dendrochronology began.

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